The 24i/0 is a computer-based digital audio hard disk recording system for Mac and Windows.

The 24i/o provides 24 balanced quarter-inch (TRS) 96 kHz analog inputs and outputs in a single rack-space. The 24i/o core system includes MOTU's next generation PCI-424 card, which provides expansion up to 96 inputs/outputs, DSP-driven mixing and monitoring, legacy I/O support, and on-board SMPTE synchronization.

In addition to all those inputs and outputs, the PCI-424 card features CueMix DSP, a flexible DSP-driven mixing and monitoring matrix that provides the same near-zero monitoring latency as today's latest digital mixers. CueMix DSP allows 24i/o users to connect keyboards, synth modules, drum machines, and even effects processors and then monitor these live inputs with no audible delay and no processor drain on the host computer's CPU. The CueMix DSP engine resides on the PCI-424 card, so it works across all interfaces connected to the card. The 24i/o can be purchased as an expander for a 2408mk3 core system, or as an independent core system which includes the PCI-424 card and AudioDesk. The core system also includes drivers for Mac OS and Windows.